What transformation method would you use to remove the transcription factor and why the given method is beneficial? I'm trying to construct an experiment with an Aspergillus sp. And I'd like my experiment to cost as less as possible

What transformation method would you use to remove the transcription factor and why the given method is beneficial? I'm trying to construct an experiment with an Aspergillus sp. And I'd like my experiment to cost as less as possible

Hey all—new to the thread. Wanted to open up a discussion as I’ve been pondering getting a Bosch dishwasher with the assumption it would like last 10+ years.
We are first time homeowners currently hand-washing. Can anyone with the experience in transitioning from one to the other speak to the efficiency, tradeoff, and ultimately any long-term savings generated by one method vs. the other? Let me know your thoughts…also factoring in the marginal time savings in having to hand-wash (sorry if this is more appropriate for another thread, but the ultimate impetus driving this decision is frugality).
Thanks! Looking forward to feedback.

SIMPLE DOCKER FULL OS

create create very simple docker full os but it needs very complex steps

1. install gnu linux for host os ex= debian stable
2. install docker in host os sh sudo apt install docker* docker-compose*
3. pull docker gnu linux os for docker linux os ex= kali linux rolling sh sudo docker pull kalilinux/kali-rolling
4. make audio fix (pulse audio fix) before start the dcoker image
   • create file named pulseaudio.socket in /home/usepulse
   • in that file write this text and save

```text default-server = unix://home/usepulse/pulseaudio.socket

Prevent a server running in the container

autospawn = no daemon-binary = /bin/true

Prevent the use of shared memory

enable-shm = false Share socket and config file with docker and set environment variables PULSE_SERVER and PULSE_COOKIE. Container user must be same as on host: ```

• run this comman in terminal sh pactl load-module module-native-protocol-unix socket=/home/usepulse/pulseaudio.socket
  1. check the docker image that we pull sh sudo docker images
  2. run the docker image with sh docker run -ti --device=/dev/dri:/dev/dri \ --privileged --cap-add=ALL --device /dev/snd \ --volume /dev:/dev -v /dev:/dev --group-add audio \ -v /varun/docker.sock:/host/varun/doc -v /:/media/prime \ --env PULSE_SERVER=unix:/home/usepulse/pulseaudio.socket \ --env PULSE_COOKIE=/home/usepulse/pulseaudio.cookie \ --volume /home/usepulse/pulseaudio.socket:/home/usepulse/pulseaudio.socket \ --volume /home/usepulse/pulseaudio.client.conf --publish=0.0.0.0:3351:3351 \ --publish=0.0.0.0:51:51 --group-add video --volume="/tmp/.X11-unix:/tmp/.X11-unix" \ --env="DISPLAY" -e XAUTHORITY=/root/.Xauthority \ --name dockerfullos-001 imageid
  3. and the kali linux docker bash terminal with appears
  4. install apps that we need ```sh apt update apt full-upgrade -y apt install nano pulseaudio* neofetch vlc* smplayer* wget uget \ cairo-dock* alsa-utils* network-manager net-tools* \ cairo-dock-plug-ins* dbus dbus-x11 thunar* chromium* \ rofi* terminator* sudo kate* kwrite* geany* geany-plugin-addons* \ aptitude* qt5-style-kvantum-themes qt5-style-kvantum-l10n \ qt5-style-kvantum libreoffice krita* gimp* kdenlive* handbrake* yt-dlp* \ isomaster* k3b* apt-utils* git htop* compiz* compiz-boxmenu* compiz-plugins* \ compizconfig-settings-manager* emerald* emerald-themes* fusion-icon* simple-ccsm* \ usbutils* lxqt* bleachbit* nmap* xrpd* openssh-server openssh-client -y

for intel gpu

dpkg --add-architecture i386 && sudo apt update apt install libvulkan1 libvulkan1:i386 mesa-vulkan-drivers mesa-vulkan-drivers:i386 vulkan-tools* -y apt install wine64 lutris*

add root password

passwd

add user

adduser username usermod -aG sudo username 9. to save the container into image - open new terminal - check the container id sh sudo docker ps -a - save the container into docker image sh sudo docker container commit --pause=false id-container nama-image:label

example

sudo docker container commit --pause=false c092aa5afec0 kali-linux:kali-linux-docker-full-os ``` it is very complex steps and needs long time :), so please make official full os for us .thanks :)

simple use case for docker full os

docker full os can make better gaming expirence

because docker full os kali linux rolling (debian testing) have newer mesa driver than debian stable , so it make game in docker full os better. the step are : - run the ali-linux-docker-full-os sh sudo docker run -ti --device=/dev/dri:/dev/dri \ --privileged --cap-add=ALL --device /dev/snd \ --volume /dev:/dev -v /dev:/dev --group-add audio \ -v /varun/docker.sock:/host/varun/doc -v /:/media/prime \ --env PULSE_SERVER=unix:/home/usepulse/pulseaudio.socket \ --env PULSE_COOKIE=/home/usepulse/pulseaudio.cookie \ --volume /home/usepulse/pulseaudio.socket:/home/usepulse/pulseaudio.socket \ --volume /home/usepulse/pulseaudio.client.conf --publish=0.0.0.0:3351:3351 \ --publish=0.0.0.0:51:51 --group-add video --volume="/tmp/.X11-unix:/tmp/.X11-unix" \ --env="DISPLAY" -e XAUTHORITY=/root/.Xauthority \ --name dockerfullos-001 imageid tipe lutris - lutris will run in host os - setting the lutris and play the game :)

docker full os can sutitude the vm

because docker full os is a native os like chroot so it has a native performance like the host, how to do it ?, it very imple

method A, acces all docker full os apps from host

```sh

run the immage

sudo docker run -ti --device=/dev/dri:/dev/dri \ --privileged --cap-add=ALL --device /dev/snd \ --volume /dev:/dev -v /dev:/dev --group-add audio \ -v /varun/docker.sock:/host/varun/doc -v /:/media/prime \ --env PULSE_SERVER=unix:/home/usepulse/pulseaudio.socket \ --env PULSE_COOKIE=/home/usepulse/pulseaudio.cookie \ --volume /home/usepulse/pulseaudio.socket:/home/usepulse/pulseaudio.socket \ --volume /home/usepulse/pulseaudio.client.conf --publish=0.0.0.0:3351:3351 \ --publish=0.0.0.0:51:51 --group-add video --volume="/tmp/.X11-unix:/tmp/.X11-unix" \ --env="DISPLAY" -e XAUTHORITY=/root/.Xauthority \ --name dockerfullos-001 imageid ``` we can use rofi or cairo-dock to lunch the docker full os apps

• using rofi
  1. type rofi --show run
  2. select the apps ex =vlc
  3. vlc docker full os apps with run directly from the host os
  4. play some movies / videos / audio with that vlc :)
• using cairo-dock
  1. type cairo-dock
  2. cairo-dcok will launch to host os
  3. launch docker full os apps fro cairo-dock menu :)

method B, we can access full gui of docker full os via xrdp

how to do it 1. run the docker image sh sudo docker run -ti --device=/dev/dri:/dev/dri \ --privileged --cap-add=ALL --device /dev/snd \ --volume /dev:/dev -v /dev:/dev --group-add audio \ -v /varun/docker.sock:/host/varun/doc -v /:/media/prime \ --env PULSE_SERVER=unix:/home/usepulse/pulseaudio.socket \ --env PULSE_COOKIE=/home/usepulse/pulseaudio.cookie \ --volume /home/usepulse/pulseaudio.socket:/home/usepulse/pulseaudio.socket \ --volume /home/usepulse/pulseaudio.client.conf --publish=0.0.0.0:3351:3351 \ --publish=0.0.0.0:51:51 --group-add video --volume="/tmp/.X11-unix:/tmp/.X11-unix" \ --env="DISPLAY" -e XAUTHORITY=/root/.Xauthority \ --name dockerfullos-001 imageid

1. edit the xrpd config in that docker full os sh nano /etc/xrdp/xrdp.ini # change to port to the open port 3351 # it will make the xrdp ip 127.0.0.1:3351 # restart xrdp server service xrdp restart # start pulse audio pulseaudo --start
2. install kdrc (rdp client) in host os
3. conect the xrdp using kdrc with the xrpd ip

```text

kdc setting

screen setting = 1024x800 audio = in this computer --- pulse audio 32bit color :) ``` 5. enjoy :) we can use docker full os with full gui in krdc :)

docker full os can use for simple cloud computing / cloud gaming

how to do it ?, it very easy 1. run the docker image, we can run multiple docker full image in single server - terminal 1 ```sh sudo docker run -ti --device=/dev/dri:/dev/dri \ --privileged --cap-add=ALL --device /dev/snd \ --volume /dev:/dev -v /dev:/dev --group-add audio \ -v /varun/docker.sock:/host/varun/doc -v /:/media/prime \ --env PULSE_SERVER=unix:/home/usepulse/pulseaudio.socket \ --env PULSE_COOKIE=/home/usepulse/pulseaudio.cookie \ --volume /home/usepulse/pulseaudio.socket:/home/usepulse/pulseaudio.socket \ --volume /home/usepulse/pulseaudio.client.conf --publish=0.0.0.0:3351:3351 \ --publish=0.0.0.0:51:51 --group-add video --volume="/tmp/.X11-unix:/tmp/.X11-unix" \ --env="DISPLAY" -e XAUTHORITY=/root/.Xauthority \ --name dockerfullos-001 imageid

change xrdp and ssh port accordingly :)

how to change ssh port

nano /etc/ssh/sshd_config

change the port to 51

- terminal 2 sh sudo docker run -ti --device=/dev/dri:/dev/dri \ --privileged --cap-add=ALL --device /dev/snd \ --volume /dev:/dev -v /dev:/dev --group-add audio \ -v /varun/docker.sock:/host/varun/doc -v /:/media/prime \ --env PULSE_SERVER=unix:/home/usepulse/pulseaudio.socket \ --env PULSE_COOKIE=/home/usepulse/pulseaudio.cookie \ --volume /home/usepulse/pulseaudio.socket:/home/usepulse/pulseaudio.socket \ --volume /home/usepulse/pulseaudio.client.conf --publish=0.0.0.0:3352:3352 \ --publish=0.0.0.0:52:52 --group-add video --volume="/tmp/.X11-unix:/tmp/.X11-unix" \ --env="DISPLAY" -e XAUTHORITY=/root/.Xauthority \ --name dockerfullos-002 imageid

change xrdp and ssh port accordingly :)

- terminal 3 sh sudo docker run -ti --device=/dev/dri:/dev/dri \ --privileged --cap-add=ALL --device /dev/snd \ --volume /dev:/dev -v /dev:/dev --group-add audio \ -v /varun/docker.sock:/host/varun/doc -v /:/media/prime \ --env PULSE_SERVER=unix:/home/usepulse/pulseaudio.socket \ --env PULSE_COOKIE=/home/usepulse/pulseaudio.cookie \ --volume /home/usepulse/pulseaudio.socket:/home/usepulse/pulseaudio.socket \ --volume /home/usepulse/pulseaudio.client.conf --publish=0.0.0.0:3353:3353 \ --publish=0.0.0.0:53:53 --group-add video --volume="/tmp/.X11-unix:/tmp/.X11-unix" \ --env="DISPLAY" -e XAUTHORITY=/root/.Xauthority \ --name dockerfullos-002 imageid

change xrdp and ssh port accordingly :)

```

each docker full os will run natively and independenly

we can acces it from the ost os with ip localhost:xrdp post (127.0.0.1:3351) with kdrc client :), we can also accest in from diffierent gnu linux computer in the same lan / wifi local network with the ip from host os that give by the router explampe 192.168.43.1 etc and xrdp port :) with kdrc

exaample xrdp

dockerfullos-001 = 192.168.43.1:3351 dockerfullos-002 = 192.168.43.1:3352 dockerfullos-003 = 192.168.43.1:3353

example of ssh

dockerfullos-001 = 192.168.43.1:51 dockerfullos-002 = 192.168.43.1:52 dockerfullos-003 = 192.168.43.1:53 enjoy the docker full os for cloud computing :), all the apps fro docker full os will run natively in kdrc in the client compuputer :), for gaming we can use emulator (ps1, ps2, psp, mame etc) , steam , lutris, wine etc :)

wine / lutris fix

wine / lutris sometime will error in docker full os, how to fix it 1. open lutris 2. setting the game with lutris 3. in the game menu , select wine regristry 4. edit the hcu ----- wine 5. add key X11 DRIVER 6. add string "UseXVidMode"="N" 7. add string UseXVidMode ----- edit the VALUE to N 8. add string "UseXRandR"="N" 9. add string UseXRandR ----- edit the VALUE to N 10. close wine
thanks :)

License

This text use creative common license

Ubuntu 20.04.1 Kernel 5.15.0-71
After coming back from a sleep state my network manager takes a few seconds to reconnect. Would like to know if I can prevent this from happening by possibly enabling NIC from going into a low power state or some other method. I have provided the output from system logs after coming out of sleep state. Please advise if there are any further details needed.
I am looking for any ideas or suggestions on overcome this issue. systemd[1]: NetworkManager-dispatcher.service: Deactivated successfully. NetworkManager[792]: [1685811431.1067] manager: sleep: wake requested (sleeping:yes enabled: yes) NetworkManager[792]: [1685811431.1068] device (enp5s0): state change: unmanaged -> unavailable (reason 'managed', sys-iface-state: 'external') NetworkManager[792]: [1685811431.2621] manager: NetworkManager state is now DISCONNECTED NetworkManager[792]: [1685811434.7142] device (enp5s0): carrier: link connected NetworkManager[792]: [1685811434.7144] device (enp5s0): state change: unavailable -> disconnected (reason 'carrier-changed', sys-iface-state: 'managed') NetworkManager[792]: [1685811434.7151] policy: auto-activating connection 'Wiredconnection 1' (d2243b54-dd72-389f-bce6-3669116dacc8) NetworkManager[792]: [1685811434.7154] device (enp5s0): Activation: starting connection 'Wired connection 1' (d2243b54-dd72-389f-bce6-3669116dacc8) NetworkManager[792]: [1685811434.7155] device (enp5s0): state change: disconnected -> prepare (reason 'none', sys-iface-state: 'managed') NetworkManager[792]: [1685811434.7156] manager: NetworkManager state is now CONNECTING NetworkManager[792]: [1685811434.7157] device (enp5s0): state change: prepare ->config (reason 'none', sys-iface-state: 'managed') NetworkManager[792]: [1685811434.7160] device (enp5s0): state change: config -> ip-config (reason 'none', sys-iface-state: 'managed') NetworkManager[792]: [1685811434.7161] dhcp4 (enp5s0): activation: beginning transaction (timeout in 45 seconds) NetworkManager[792]: [1685811442.7904] dhcp4 (enp5s0): state changed new lease, address=192.168.xx.xx dbus-daemon[790]: [system] Activating via systemd: service name='org.freedesktop.nm_dispatcher' unit='dbus-org.freedesktop.nm-dispatcher.service' requested by ':1.5' (uid=0 pid=792 comm="/ussbin/NetworkManager --no-daemon " label="unconfined") NetworkManager[792]: [1685811442.7918] device (enp5s0): state change: ip-config -> ip-check (reason 'none', sys-iface-state: 'managed') NetworkManager[792]: [1685811442.8023] device (enp5s0): state change: ip-check -> secondaries (reason 'none', sys-iface-state: 'managed') NetworkManager[792]: [1685811442.8025] device (enp5s0): state change: secondaries -> activated (reason 'none', sys-iface-state: 'managed') NetworkManager[792]: [1685811442.8028] manager: NetworkManager state is now CONNECTED_LOCAL NetworkManager[792]: [1685811442.8030] manager: NetworkManager state is now CONNECTED_SITE NetworkManager[792]: [1685811442.8031] policy: set 'Wired connection 1' (enp5s0)as default for IPv4 routing and DNS NetworkManager[792]: [1685811442.8036] device (enp5s0): Activation: successful, device activated. NetworkManager[792]: [1685811442.8039] manager: NetworkManager state is now CONNECTED_GLOBAL systemd[1]: NetworkManager-dispatcher.service: Deactivated successfully.

What is relationship abuse?
Relationship abuse is a pattern of abusive and coercive behaviors used to maintain power and control over a former or current intimate partner. Abuse can be emotional, financial, sexual or physical and can include threats, isolation, and intimidation. Abuse tends to escalate over time. When someone uses abuse and violence against a partner, it is always part of a larger pattern of control.
Warning signs of abuse:

• Do you feel nervous around your partner?
• Do you have to be careful to control your behavior to avoid your partner’s anger?
• Do you feel pressured by your partner when it comes to sex?
• Are you scared of disagreeing with your partner?
• Does your partner criticize you, or humiliate you in front of other people?
• Is your partner always checking up on you or questioning you about what you do without your partner?
• Does your partner control where you go or check the mileage on your car?
• Does your partner repeatedly and wrongfully accuse you of seeing or flirting with other people?
• Does your partner tell you that if you changed, they wouldn’t treat you like this?
• Does your partner’s jealousy stop you from seeing friends or family?
• Does your partner make you feel like you are wrong, stupid, crazy, or inadequate?
• Has your partner ever scared you with violence or threatening behavior?
• Does your partner throw or break objects to intimidate you?
• Does your partner make you feel scared by driving too fast and refusing to slow down when you ask?
• Does your partner say, “I will kill myself if you break up with me” or “I will hurt/kill you if you break up with me”?
• Does your partner make excuses for the abusive behavior? For example: saying, “It’s because of alcohol or drugs,” or “I can’t control my temper,” or “I was just joking”?
• Does your partner brag about bullying or harming others or animals?
• Has your partner abused or killed your animals?
• Does your partner impose stereotypical gender roles or invalidate your gender identity?

​
Types of abuse:
Physical abuse is any intentional, unwanted contact with you or something close to your body, or any behavior that causes or has the intention of causing you injury, disability, or death.

• ^{Scratching, punching, biting, strangling, choking, or kicking.}
• ^{Throwing items at you like a phone, book, shoe, or plate.}
• ^{Pulling your hair.}
• ^{Pushing or pulling you, or forcibly grabbing your clothing.}
• ^{Threatening to use or using a gun, knife, box cutter, bat, mace, or other weapon against you.}
• ^{Touching any part of you without your permission or consent.}
• ^{Forcing you to have sex or perform a sexual act.}
• ^{Grabbing your face to make you look at them.}
• ^{Preventing you from leaving or forcing you to go somewhere.}

Emotional abuse & verbal abuse includes non-physical behaviors such as threats, insults, constant monitoring or “checking in,” excessive texting, humiliation, intimidation, isolation, or stalking.

• ^{Calling you names or putting you down.}
• ^{Telling you what to do or wear.}
• ^{Yelling or screaming at you.}
• ^{Intentionally embarrassing you in front of others or starting rumors about you.}
• ^{Preventing you from seeing or communicating with friends or family, or threatening to have your children taken away from you.}
• ^{Damaging your property (throwing objects, punching walls, kicking doors, etc.})
• ^{Using online communities or communications to control, intimidate, or humiliate you.}
• ^{Blaming abusive or unhealthy behavior on you or your actions.}
• ^{Being jealous of outside relationships or accusing you of cheating.}
• ^{Stalking you or your loved ones.}
• ^{Threatening to harm you, your pets, or people in your life.}
• ^{Threatening to harm themselves to keep you from ending the relationship.}
• ^{Gaslighting you by pretending not to understand or refusing to listen to you; questioning your recollection of facts, events, or sources; trivializing your needs or feelings; or denying previous statements or promises.}
• ^{Making you feel guilty or immature when you don’t consent to sexual activity.}
• ^{Threatening to expose personal details, such as your sexual orientation or immigration status.}

Sexual abuse refers to any behavior that pressures or coerces someone to do something sexually that they don’t want to do. It can also refer to behavior that impacts a person’s ability to control their sexual activity or the circumstances in which sexual activity takes place, including oral sex, rape, or controlling reproductive methods and choices.

• ^{Unwanted kissing or touching.}
• ^{Unwanted rough or violent sexual activity.}
• ^{Refusing to use condoms or restricting someone’s access to birth control.}
• ^{Preventing someone from using protection against sexually transmitted infections (STIs}.)
• ^{Sexual contact with someone intoxicated from drugs or alcohol, unconscious, asleep, or otherwise unable to give clear and informed consent.}
• ^{Threatening, pressuring, or otherwise forcing someone to have sex or perform sexual acts.}
• ^{Using sexual insults toward someone.}

Financial abuse often operates in more subtle ways than other forms of abuse, but it can be just as harmful to those who experience it.
Modern conditions of stark economic inequality mean that financial security is directly tied to our health and wellbeing. No one has the right to use money or how you choose to spend it to control your actions or decisions, and no one should control your ability to work.

• ^{Giving you an allowance or monitoring what you buy.}
• ^{Depositing your paycheck into an account you can’t access.}
• ^{Preventing you from seeing shared bank accounts or records.}
• ^{Forbidding you from working or limiting the hours you do.}
• ^{Preventing you from going to work by taking your car, keys, or other mode of transportation.}
• ^{Getting you fired by harassing you, your employer, or your co-workers.}
• ^{Hiding or stealing your student financial aid check or other financial support.}
• ^{Using your social security number to obtain loans without your permission.}
• ^{Using your child’s social security number to claim an income tax refund without your permission.}
• ^{Maxing out your credit cards without permission.}
• ^{Refusing to provide you with money, food, rent, medicine, or clothing.}
• ^{Using funds from your children’s tuition or a joint savings account without your knowledge.}
• ^{Spending money on themselves while preventing you from doing the same.}
• ^{Giving you presents or paying for things with the expectation of something in return.}
• ^{Using financial circumstances to control you.}

Digital dating abuse is the use of technologies like texting and social media to bully, harass, stalk, or intimidate a partner. This behavior is often a form of verbal or emotional abuse, conducted online.

• ^{Telling you who you can or can’t follow or be friends with on social media.}
• ^{Sending you negative, insulting, or threatening messages or emails.}
• ^{Using social media to track your activities.}
• ^{Insulting or humiliating you in their posts online, including posting unflattering photos or videos.}
• ^{Sending, requesting, or pressuring you to send unwanted explicit photos or videos, sexts, or otherwise compromising messages.}
• ^{Stealing or pressuring your to share your account passwords.}
• ^{Constantly texting you or making you feel like you can’t be separated from your phone.}
• ^{Looking through your phone or checking up on your pictures, texts, and phone records.}
• ^{Using any kind of technology (such as spyware or GPS in a car or phone to monitor your activities.})

Stalking occurs when someone watches, follows, or harasses you repeatedly, making you feel afraid or unsafe.

• ^{Showing up at your home or workplace unannounced or uninvited.}
• ^{Sending you unwanted texts, messages, letters, emails, or voicemails.}
• ^{Leaving you unwanted items, gifts, or flowers.}
• ^{Calling you and hanging up repeatedly or making unwanted phone calls to you, your employer, a professor, or a loved one.}
• ^{Using social media or technology to track your activities.}
• ^{Spreading rumors about you online or in person.}
• ^{Manipulating other people to investigate your life, including using someone else’s social media account to look at your profile or befriending your friends in order to get information about you.}
• ^{Waiting around at places you spend time.}
• ^{Damaging your home, car, or other property.}
• ^{Hiring a private investigator to follow or find you as a way of knowing your location or movements.}

---
Avoid victim blaming
Why is it dangerous?
^{Victim-blaming attitudes marginalize the victim/survivor and make it harder to come forward and report the abuse. If the victim/survivor knows that you or society blames survivors for abuse, they will not feel safe or comfortable coming forward and talking to you.
Victim-blaming attitudes also reinforce the manipulative tactics that abusers use to control their partner; abusers tell survivors that it is their fault this is happening. Committing violence is always the choice of the person who is abusing. It is NOT the victim/survivor’s fault or responsibility to fix the violence that an abuser is committing against them. By engaging in victim-blaming attitudes, society allows abusive people to perpetrate relationship abuse or sexual assault while avoiding accountability for those actions.
Victim-blaming attitudes prevent society from acknowledging and changing toxic masculinity and rape culture.}
Where does it come from?
^{In order to stop victim-blaming, it is helpful to understand why it occurs in the first place. One reason that people blame a victim/survivor is to distance themselves from an unpleasant occurrence. This gives a false sense that this could not happen to them. By labeling or accusing the victim/survivor, others can see the victim/survivor as different from themselves. People use the Just World theory, Invulnerability theory, and Assumptive World theory in an attempt to feel like they have control over situations where they do not have control. People reassure themselves by thinking, “Because I am not like the victim/survivor, because I do not do XYZ, this would never happen to me.” We need to help people understand that a survivor’s actions do not contribute to a perpetrator’s (those that choose to harm decision to commit relationship abuse and sexual violence. It is our responsibility as members of society to support survivors and hold abusers accountable.})
What does victim blaming look like?
^{Common Victim Blaming Statements:}

• ^{“The victim provoked him”}
• ^{“They both have problems”}
• ^{“The victim shouldn’t have married him after”}
• ^{“The victim was drunk”}
• ^{“They can’t be abused, because they are in an LGBTQI+ relationship”}

^{Example of Victim-Blaming Attitude:}

• ^{“She must have provoked him into being abusive. They both need to change.”}

^{Reality: This statement assumes that the victim is equally to blame for the abuse, when in reality, abuse is a conscious choice made by the abuser. Abusers have a choice in how they react to their partner’s actions. Options besides abuse include: walking away, talking in the moment, respectfully explaining why an action is frustrating, breaking up, etc. Additionally, abuse is not about individual actions that incite the abuser to hurt the victim/survivor, but rather about the abuser’s feelings of entitlement to do whatever the abuser wants to their partner. When friends and family remain neutral about the abuse and say that both people need to change, they are taking away responsibility from the perpetrator (those that choose to harm}, ^{thereby colluding with/supporting the abusive partner and making it less likely that the survivor will seek support.})
^{Victim Blaming in Language:One of the biggest sources of victim blaming is the way we talk about it. Language surrounding abuse and sexual assault immediately puts our attention on the victim instead of the perpetrator. This is a demonstration developed by Julia Penelope and frequently used by Jackson Katz to show how language can be victim blaming:}

• ^{John beat Mary; This sentence is written in active voice. It is clear who is committing the violence.}
• ^{Mary was beaten by John; The sentence has been changed to passive voice, so Mary comes first.}
• ^{Mary was beaten; Notice that John is removed from the sentence completely. Our attention is completely focused on Mary.}
• ^{Mary is a battered woman; Being a battered woman is now part of Mary’s identity. John is not a part of the statement, and he will not be held accountable for his choice to abuse.}

^{As you can see, the focus has shifted entirely to Mary instead of John, encouraging the audience to focus on the survivor’s actions instead of the perpetrator’s actions. The solutions regarding prevention become focused on what Mary can do differently, not on what John can do differently, and not on how society creates a culture that supports John’s behavior.}
What can I do about it?

• ^{Challenge victim-blaming statements when you hear them}
• ^{Do not agree with abusers’ excuses for why they abuse}
• ^{Let survivors know that it is not their fault}
• ^{Hold abusers accountable for their actions: do not let them make excuses like blaming the victim, alcohol, or drugs for their behavior}
• ^{Acknowledge that survivors are their own best experts and provide them with resources and support}
• ^{Recognize that victim-blaming can take on many forms and manifest in unique ways, which can be rooted in racist, sexist, and/or homophobic attitudes present in society.}
• ^{Avoid victim blaming in the media}
• ^{Reframe the question “Why does the victim stay?” to “Why does the perpetrator (those that choose to harm abuse?” See Barriers to Leaving an Abusive Relationship}
• ^{Understand the frequently asked questions that often interrupt accountability.}
• ^{See Tips for Media Professionals for information on how you can re-frame victim-blaming language.}

^{Remember if you are aware of abusive behavior and do not speak out against it, your silence communicates implicitly that you see nothing unacceptable taking place.\})
---
Additional resources for those experiencing abuse in relationships:

• Plan for safety if you are experiencing abuse.
• Learn more about how to support a loved one who is experiencing abuse.
• Educate yourself about barriers to leaving abusive relationships.

https://www.loveisrespect.org/ / Call 1-866-331-9474 / Text LOVEIS to 22522
https://www.thehotline.org/ / Call 1.800.799.SAFE (7233) / Text START to 88788
https://stoprelationshipabuse.org/

Timestamp: https://imgur.com/a/UbfdiUw
Brand new set of Axil GS Extreme 2.0 Ear Pros
SV/TV: $85 Shipped
Looking for: Ranger Green D3CRM, K19 Gen 3 RG, Holosun EPS Carry Green 2moa, Unity Hot Button Surefire 1913 and will add cash on my end if necessary
Payment, Shipping, & Transaction Info: NO G&S due to tax changes with paypal.
1 - Payment Methods: Paypal F&F, Venmo F&F, and Cashapp with NO NOTES IN DESCRIPTIONS. Use an emoji for Venmo.
2 - I use Pirate Ship to create labels and will provide tracking as soon as possible. Lower 48 only, no HI or AK.
3 - NO TRANSACTION NOTES OF ANY KIND.
4 - Call "dibs" to secure what you want. "Dibs" will have priority over "Interested" or "PM sent".
5 - Unless something is marked as SOLD, it is still available. Call 2nd Dibs, 3rd Dibs, etc. in a comment below.
6 - Buyer will have 1 hour to send funds. Item(s) will be passed to next in line if no funds are received within an hour. If you need more time just let me know.

I’m not a native english speaker so sorry in advance if this post contains any grammatical errors. I’m neither a doctoresearcher or a science guy so I’ll try to alway provide some reference but be aware that the information that follows may be misinterpreted or simply not correct.
The aim of this post is to understand what is the consensus and the consolidated evidence on PE, and what information we should expect from a clinician/doctor (so you probably won’t find any new therapy you have never heard of). I’m curious and wanted to know a little bit more about this topic since I think there is a lot of misinformation and common beliefs that are rooted but are not backed up by any scientific evidence and even doctors/clinician sometimes are not educated enough on the topic to provide any actual help.Don’t expect anything super well summarised, this is going to be more of a copy and paste work.
Following the Hierarchy of Evidence in medical research meta-analysis, systematic reviews and practice guidelines provide the highest quality of evidence, I focused on the guidelines because I think that are more practical and have more reach to doctors/clinician that are not expert in the field. I found the ISSM’s (International Society of Sexual Medicine) guidelines to be the most complete guidelines on the topic. The International Society for Sexual Medicine (ISSM) is a medical society devoted to the study of the medicine of human sexuality. It publishes two journals, The Journal of Sexual Medicine (where the guidelines were published, IF for 2022-2023 is 3.9), and the open-access Sexual Medicine Reviews. The last update of the guidelines I found is from 2013/2014, American Urology Association has its own guidelines updated in 2020, they are not specifically focused on just PE but on Disorders of Ejaculation, mainly PE and DE (Delayed Ejaculation). The AUA’s guidelines focus mainly on the management of the patient and the pharmacotherapy and don’t give many indications about the main theories on PE’s causes.

Definition from ISSM

The panel proposed a unified definition of LPE (Long Term PE) and APE (Acquired PE):
It “is a male sexual dysfunction characterized by:

• ejaculation that always or nearly always occurs prior to or within about 1 minute of vaginal penetration from the first sexual experience (LPE), OR a clinically significant reduction in latency time, often to about 3 minutes or less (acquired premature ejaculation);
• the inability to delay ejaculation on all or nearly all vaginal penetrations; and
• negative personal consequences, such as distress, bother, frustration, and/or the avoidance of sexual intimacy.”

It also identify additional subtypes: VPE (Variable PE) that occurs irregularly and inconsistently and SPE (Subjective PE) a subjective perception of PE but with the IELT in the “normal range” (so a IELT >= 3 mins, the median IELTs from 2 studies is 5.4 minutes and 6 minutes respectively).
The estimated prevalence of APE and LPE is around 5%, the prevalence of VPE and SPE is higher but these definitions are relatively new so there isn’t a lot of data.

Etiology (form ISSM’s guidelines)

I start by saying that of all the biological factors that have been proposed to explain PE, none has been confirmed in large scale studies, nonetheless some studies are really interesting.
Hypersensitivity of the Glans -> in a small study was found that patient with PE have hypersensitivity and hyperexcitability of the glans via performing somatosensory evoked potentials (brain responses to sensory stimuli). A similar study not cited in ISSM’s Guidelines is Zhang 2009 that found in a small cohort that patients with PE have on average a higher number of dorsal penile nerve branches. Disturbances in central serotonergic neurotransmission -> We know that Serotonin is the main neurotransmitter involved in the control of ejaculation, but in humans specific subtypes of 5-HT (serotonin) receptors involved in PE have not yet been identified. SSRIs work but SSRIs treatment activates many different postsynaptic subtype receptors so we don’t know specifically how it works. In the spinal cord, specific neurons have been found to be essential to ejaculatory reflex in rats and there is also preliminary evidence that such a neural organisation also exists in humans. Genetics of PE -> Some studies have investigated gene polymorphism of receptors of serotonin and oxytocin. Results of the studies are mixed, men with some genetic variants may be predisposed to develop PE but more studies are needed. Hormones imbalances -> Hyperthyroidism has no role in LPE and has been found to be associated with APW in extremely few patients. Recent studies on large population indicate that the endocrine system is involved in the control of ejaculatory function: prolactin in the lowest quartile levels is associated with APE and anxiety symptoms and higher testosterone levels aìcorrelate with PE, while lower androgenization is related to delayed ejaculation. Anyway the guidelines state that both low PRL and relatively high testosterone levels cannot be considered etiologies of PE. Prostatitis -> Prostatic inflammation and chronic bacterial prostatitis have been reported as common findings in men with APE. For the guidelines though there is insufficient evidence to support routine screening of men with PE for this condition. Erectile dysfunction -> For the guidelines ED is unlikely as a comorbidity or etiological factor for LPE, there is data to support that APE is associated with PE. Psychological factors -> there has been limited research on causality; most studies can only report association. It is plausible that psychological factors may lead to PE or vice versa, it is likely that for many men the relationship is reciprocal with either PE or the other factor causing exacerbation of the other.

Psychological/Behavioral interventions

There is not much to say on this topic, well designed studies on behavioural interventions are scarce, apart from the squeeze/start-stop technique other interventions are correct breathing and relaxation of the muscles to control excitement (discussed a lot in this community).

Pharmacological Treatment (from ISSM guidelines)

Dapoxetine: it is a rapid acting and short half-life SSRI with a pharmacokinetic profile supporting a role as an on demand treatment for PE. In randomised controlled trials, dapoxetine 30mg or 60mg taken 1-2 hours before intercourse is more effective than placebo from the first dose resulting in a 2.5 and 3.0 fold increase in IELT. Treatment-related side effects were uncommon, dose dependent and included nausea, diarrhoea, headache and dizziness: they were responsible for study discontinuation in 4% (30mg) and 10% (60mg) of subjects.
Off-Label SSRIs and TCAs: Daily treatment with off-label paroxetine 10-40 mg, clomipramine 12.5-50 mg, sertraline 50-200 mg, fluoxetine 20.40 mg and citalopram 20.40 mg is usually effective in delaying ejaculation. A meta-analysis of published data suggests that paroxetine exerts the strongest ejaculation delay, increasing IELT approximately 8.8-fold over baseline. SSRIs should be avoided in men with a history of bipolar depression.
Topical Local Anesthetics: lidocaine and/or prilocaine as a cream, gel, or spray is well established and is moderately effective in delaying ejaculation, data suggest that diminishing the glans sensitivity may inhibit the spinal reflex that are responsible for ejaculation. PSD502 (Fortacin in Europe) , a lidocaine-prilocaine spray applied 5 minutes before intercourse is associated with a 6.3-fold increase in IELT. There were minimal reports of penile hypoanesthesia and transfer to the partner due to the unique formulation of the compound.
PDE5i: SIldenafil, Tadalafil, Vardenafil, are effective treatments for ED, there is some evidence to support the efficacy and safety of off-label on demand or daily dosing of PDE5i in the treatment of LPE in men with normal erectile function but further evidence based research is encouraged to understand conflicting data.

Other pharmacological treatments

Tramadol: Tramadol has been investigated as a potential off.label therapy for PE, with several studies demonstrating efficacy, the mechanism of action is not completely understood. It may be considered when other therapies have failed because of the risk of addiction and side effects.
Oxytocin: Intraventricular administration of oxytocin antagonist inhibits sexual behaviour in animal studies, however in one human study, an oxytocin antagonist failed to clinically or statistically improve IELT, further human studies are necessary.
Alpha1-adrenoceptor antagonists: not mentioned in ISSM’s guidelines but in the AUA’s. There are not good quality studies on these drugs, Silodosin seems the most effective one. From AUA

Other treatments

Cryoablation and Neuromodulation of the Dorsal Penile Nerve: invasive and irreversible procedure, which is associated with an increase in the IELT. However, safety of this treatment modality needs to be determined before this procedure can be recommended for treating patients.
Intracavernosal Injection for PE: There is limited evidence regarding the efficacy of intracavernosal vasoactive drug injection for the treatment of PE.
Injections with hyaluronic acid in the glans: a study cited by AUA found a 2.6 fold increase vs 1.1 placebo in ELT, AUA recommends that surgical intervention for PE only to be considered in the context of ethical board approved trial for patients who have failed or cannot tolerate alternative management strategies for PE.

Preclinical studies for PE

Several agents have been studied in animal models for treating PE: a potent SSRI (DA-8031) significantly inhibited ejaculation in rat models. There are some human studies after 2013 (so after the publication of the guidelines) but I can’t understand in which phase the drug is and if the possible therapy with this SSRI is on-demand or not and the drug is not mentioned in AUA’s guidelines and I don’t understand why.
Modafinil, used in the treatment of narcolepsy, produced significant delay in ejaculation in rat models. Can’t find a lot of studies after 2013, in the AUA’s guidelines form 2020 it’s reported that in an uncontrolled pilot study of on-demand modafinil it’s reported a modest but significant two-fold increase in IELT.
Injection of botulinum toxin in the bulbospongiosus muscle, from AUA’s guidelines: the toxin that blocks neural transmission when injected into the muscle. But studies for PE were discontinued due to lack of efficacy in interim analysis.
Oxytocin antagonists (from AUA’s guidelines): Cligosoban is a oxytocin receptor antagonist that in a RCT double blind trial showed statistically significant effect of LPE men (3.6 fold increase in ELT vs 1.8 for placebo).

Further study and potentially development of agents with different distribution is required to determine if these therapies will have a role in PE management in the future.

Apart from the usual (G)ARCH volatility models, there's this very basic method of estimating dynamic volatility called "rolling/moving variance" or "rolling window model of the conditional variance" [Guidolin & Pedio, 2018, sec. 5.2.1]. It's completely analogous to the rolling average and is defined as:

var[t] = avg{r[t]^2, r[t-1]^2, ..., r[t-W+1]^2} 

Here, {r[t]} are stock returns. Essentially, variances are computed for overlapping windows {r[t-W+1], ..., r[t-1], r[t]} assuming zero mean.
This seems simple enough, but I'm unable to find who invented this method. Or who first used it? Who explained why it can be used as a variance estimator?
Sure, it's a special case of ARCH with no additive constant and equal weights. However, it was known before ARCH (which appeared in 1982):

• [Officer, 1973] uses "1-year standard deviation of the monthly market factor, estimated as a monthly moving series". He further explains that "the [volatility] series was obtained by estimating the standard deviation of the market factor for the first 12 months of data, then the first month was dropped and the 13th month added to obtain a new estimate". Officer doesn't explain why he estimates variance/volatility like this, as if this approach was common knowledge.
• [Merton, 1980, Appendix A] uses non-overlapping windows and thus treats variance within each window as constant. This is a similar, but different approach, since windows don't overlap here.

Does anyone know who introduced this way of estimating time-varying volatility?

References

1. Guidolin & Pedio, "Time series for financial applications", 2018.
2. Officer, R.R., "The variability of the market factor of the New York Stock Exchange", 1973.
3. Merton, R.C., "On estimating the expected return on the market", 1980.

Based on Dave's new video (https://youtu.be/7WwqV0cBoGA).
There is a major problem here and that is the idea that we need to achieve energy abundance and international cooperation before we get ASI. Essentially, you are saying that if we don't achieve utopia before we get ASI then we are almost certain to get dystopia and extinction. Given that there is an absolutely zero percent chance we get there in the next 20 years and a practically zero chance we get there in the next 100 years, does this mean that the only solution is to completely stop all progress on AI immediately before we reach the typing point where the AI can improve itself?
We are already very close to, and have perhaps already passed, the point at which an AI is capable of being given agency and doing its own AI research. Granted, the most powerful and capable models are affiliated with large companies as they cost a lot to run but both anthropic and OpenAI were small shops before they produced their big model and Google and Microsoft invested in them rather than built them. So it is clear that a small shop can build a powerful AI and we are shrinking these models everyday through better chips and better algorithms. At some point in the very near future you will be able to build an AGI on a system that is affordable for a single household (i.e. costs as much as a house).
If you combine these two ideas, that we need to achieve utopia before we get ASI and that we'll be capable of building ASI (or at least starting it) within the next decade, the only possible solution is to institute a complete ban on computer progress. This would likely also need to be a ban on physics and other science as those can lead to breakthroughs that make computing cheaper and they will need more compute power to continue advancing. A ban this powerful, and world wide, will of necessity be one of the most ubiquitous police states in the world and will rival North Korea. The additional problem is that once you give the government the power to regulate any science or research, including hobbyists, then you have given so much power to the state that corruption is inevitable.
This world, where we stop AI now, is the dystopia we fear. We are proposing to build the very monster we fear in order to save us from itself.
I don't think it is possible to build the utopia until we have the AGI (or something close to it). I look at Marx for this. The economics of a society shape its power system. In a capitalist economy you have a few factors which shape or economy. The first is the division between labor and capital. The tools to produce value are expensive and difficult to obtain. Initially you needed massive factories and tracts of land. This limited ownership to all but the very few. For everyone else to survive, they needed to hitch themselves as laborers to one of these owners.
As time has increased technology has grown to reduce the amount of capital you need to create wealth. The FANG companies were funded by small entrepreneurs with home equipment. They did, however, have to attract large investments to grow. Silicon valley maintains this trend where amazing new tools (and really shitty ones) are created by individuals but that big capital is needed to rev them up. This is why the capitalist system maintains itself.
AGI is extra powerful because it allows a single person to do so much. (This is assuming AGI that is capable of replacing human labor but still needs human initiative.) This becomes more true as we move AI from million dollar super computers into people's homes. The individual will be able to compete on an unheard of level. The Internet tools that we all use will be replicable by any individual. Big investors won't be able to keep up as by the time they finish the paperwork to invest a new competitor has already shot up. Additional advances, like ubiquitous 3D printing, configurable factories, and eventually nano-replicators, will help eliminate the grip of physical manufactures.
As the capability gap between the investor class and the working class shrinks, the investor class will lose their power. A post-scarcity world will emerge, though it will likely involve many bloody conflicts. AGI is a primary tool we need to make this happen and so it, necessarily, must come before the utopia but after it. A hyper intelligent AI will be able to assess the situation and help us navigate towards the better society.
This is why, my theory is that the best method to build a utopian society is to get powerful AI into the hands of as many people as possible.

Below is a list of the deltas awarded in this post.
Please note that a change of view is not necessarily a reversal, and that OP awarding a delta doesn't mean the conversation has ended.
For a full explanation of the delta system, see here.

Deltas from OP SignificantAd2222

• 1 delta from OP to hungryCantelope for "Reasonable person is a standard legal term which is intentinoally abstract since a more specific def..."
  
• 1 delta from OP to Ansuz07 for "If they don’t inform you of the risks, they yes - they are liable. Medical malpractice suits are l..."
  
• 1 delta from OP to Alexandur for "Yes, the severity of the damage is going to be a relevant factor come litigation time."
  
• 1 delta from OP to Nrdman for "Weed is not typically sold with nicotine lol. In the proposed situation, labeling would not include ..."
  

Deltas from Other Users

None yet.

Clarification Note

- This is a new post format I am trying out on different platforms.
- I am going to be more direct in this post, that said if you do not fit the criteria in my post please do not contact me unless you're seeking friendship.
- I am interested in a sugaring dynamic added to BDSM, this takes time. If you want a buyer or a John please avoid me.
- If you're out of country, refer to the first note.
​
Special NoteIt's Sunday. If you're still hung over, and or still going to have your fun take care of yourself - eat some protein and drink plenty of water. If you have an extreme headache and over the counter meds aren't helping and you're still wanting to be a masochist cayenne pepper mixed with warm water will do the trick. Aside from that, if you do not read this entire post I'll know.
​
Side Note
If you're curious to get to know me, just ask.
​

All about what I am looking for and offer

- Age range 18 - 28.
- Good Hygiene, you can take care of yourself mentally and physically.
- CIS Female
- Body Type Preference: Slim/Petite to average and or curvy.
- Switches, Switchtastic times are the best times. Slaves, littles, and subs are nice as well though.
- Positive influence, that goes both ways in expectancy.
- Conversation, if you hit me with the ole buy meh nudes I will meme you. If you hit me with with the ole Daddy comment imma hit you with the worst site I can think of which is currently omegle.
- Gamers - preferably a female but if you're a guy and do competitive gaming I am open to a mutually beneficial arrangement in the realm of gaming.
- You're in Louisiana, yes this unfortunately dead state it seems. But if you're in the surrounding states I am open to it.
- Adrenaline junkie - We only have one life, might as well test it a little by zip lining or jumping out of a plane together.
- An Adult, if you've attempted to meet and or have met someone from here or other platforms you know what I am speaking of.
- BDSM, be into it and or open to being guided in it.
- Kinky Oranges, this better be in your first message to me.
​
Dynamics I align with best
Equal Ownership
Master to slave
Daddy to little
Owner to pet
Care Giver to little
​
Payment Methods I work with
- Google pay (primary option)
- Amazon Wish List (secondary only, meant for gifts)
- Gift Cards (secondary only, meant for gifts)
- The Dollar (because reddit doesn't like the C word for some reason) (primary option)
- Ko-fi's site (primary option)
- CA (primary option)
- Open to other alternatives if asked.
​
How my arrangement works
This is meant to be long term, it's going to take time to establish a connection and trust. If you're expecting to talk to me for 2 hours and get 400$ it's not going to happen. Ironically, I was scammed last month out of a starting trial period with a real person for 400$ after speaking to that person for two weeks. I don't always take two weeks to get to know someone but it depends on the vibes. For additional context, I believe this platform does not like the discussion of allowances in post so I will be omitting the allowance amounts from all stages.
​
Consideration
Where you and I will be when you decide to reach out to me. We'll get to know the other from our vanilla life to our experience in the kink life, learning of the others limitations and interest. At some point I am going to prompt us both to do a SFW verification via call and or picture exchange. Silverware is a thing so don't be surprised if I prompt for something random. I will likely prompt for at least 1 - 2 calls, if we mesh then we mesh. If we don't then we can agree for friendship or parting ways.
​
If in Louisiana - The above will take place first, then an initial meet to a restaurant will be prompted. I will cover all expenses, and if needed support travel. If we mesh we mesh, if we do not mesh we can part ways and or maintain a friendship.
Trial
In this stage, we've agreed we mesh in chemistry and wish to push forward.
This is a two week trial. In this trial a light core dynamic will begin, I will take on the Dominant role regardless if you're a switch or not. I expect moderate time to be given while we continue to engage with the other. This will include a bare basic rule system to give a glimpse of interest in real time, light NSFW play, and establishing further trust in the other.
​
If in Louisiana - The above will likely be online. Meets to restaurants and or events will be expected. This is SFW.
​
Training
In this stage, we've agreed we still mesh in kink and chemistry and wish to push forward.
This will continue into a two week allowance arrangement with occasional gifts. The allowance will be raised from the trial period. This stage will consist through a time span of 2 - 6 months. I will maintain the Dominant role at least for the start dependent on our interest for dynamics. This will be a true start to a generic Dom / sub dynamic that will merge into others as time passes with moderate rules and guidance both in kink and your life as I wish to push you toward your goals. NFSW and SFW.
​
If in Louisiana - I do expect regular meets which will be determined together, it will be SFW and NSFW for scene play and or sessions along with outtings. Sadistic Pineapples better be in your first message.
​
If out of state - Semi regular to regular meets are expected. Travel will be covered. First meet will be SFW if you are uncomfortable for anything beyond.
​
Formal
We've established that we're both in it for the long run. We will either maintain two week allowance periods or weekly allowance periods dependent on what we're looking for. Gifts are regular. A true formality collar is introduced, if you're interested I also have a role play based BDSM contract. A true dynamic will be established between us with my full list of rules, guidance and scene play.

Tl;Dr: while Unity is sending the update of your world to vrchat's servers, spam updates to your world's description on vrchat's site the entire time. I'd do this by adding a period to my description and then saving changes, rinse and repeating until Unity was done. There's a window of time while Unity updates your world that you have to catch with a website update at the same time, so the faster you save changes on the site, the better chance you have at hitting that window.
 
That title is confusing. Anyways, I have been testing non stop how to fix this bug. It's the one where if you upload a change to your world, the Updated date for your world's info doesn't change to Today's date, resulting in you not getting the "Updated Recently" tag or showing up in the Updated Recently category. And I think I have finally found a solution.
I have tested this four different times and it has worked all four times so it's hard for me to label it as coincidence. Especially since I can upload my world 20 times in a row without doing this thing and it never change the Updated date but it'll change it on my first or second upload whenever doing this method (it not working on my first attempt has an explanation further below).
Here's the possible fix: I would see commonly that people used to mention how just putting a period in your world's description and saving it on the site would change the Updated date but that didn't really seem to work anymore. So I did a thing. I spammed period updates in my description on the site WHILE my world was in its final leg of uploading. At some point, during the upload process, there is a small window where if you update your world's description on the site at the same time, it'll change your Updated date. I don't know where that window is and I have missed it a couple of times but it always works on my second attempt, so you have to be fast. The slower you are, the more times it might take you because you miss that window.
Basically, what I would do is I'd let Unity compile the upload and then when I got to the upload screen (where I can name my world and give it a description, etc), I'd put unity on one half of my screen and the vrchat site on the other half of my screen, ready to edit my world's description. I'd then click Update on my world (or upload, whatever it says, I forget) in Unity, and then immediately go over to the website and start spamming period updates. I'd add a period to the description, quickly click Save Changes, then add another period to the description, quickly click Save Changes, repeat repeat repeat, I'd do this as fast as I could until Unity was finished with it's upload (about 30-60 seconds). I'd then delete all of the periods, save changes, and hopefully the date had changed. Twice it changed on the first attempt. Two other times it didn't because I must've not saved changes during the window of time I needed to, but then I'd catch that window on my second attempt. It might take you three or four tries, but the faster you go, the more chances you have of hitting that window.
Please let me know if this works for you or if not and how many times you tried in a row

[[email protected]](mailto:[email protected]) ---------------------------- Sometimes, people get lucky. You might only need to hack your teacher’s, lecturer’s or professor’s email to change the grade. It all depends on how thorough you are with making research on the job. That is why we advise that you HIRE [J](mailto:[email protected])essica the proper professional hacking tools and expertise to get the job done for you.
What is PowerSchool?
PowerSchool SIS is a Student Information System in form of web-based application designed for schools. It makes it easy for parents and students to log into a secure account from home, school or work; access real-time assignments, grades and attendance; request reports, email teachers and do more.
PowerSchool tracks student records and progress. School administrators and teachers enter data into the system, where it is stored. When you need to access the information, you can perform searches and run reports. Parents can monitor their children’s progress and learn about school activities.
How Secure is PowerSchool?
PowerSchool hosting is delivered through Microsoft Azure cloud-based technology. All cloud models are susceptible to threats. However, Cloud Computing centralizes applications and data making it very easy to detect hacking if any of the data is tampered with. Though it is secured to some extent it can still be hacked by a very good hacker.
How to Hack PowerSchool Without Being Noticed
Trying to gain unauthorized access to PowerSchool SIS could be very stressful and dangerous (if caught). It is always important to know the status of the college or University’s cyber-security, database protocol and security review periods when trying to change grades or collect data from the school’s database when the grades are uploaded otherwise change of grades might not need hacking the whole school’s system.
Sometimes, people get lucky. You might only need to hack your teacher’s, lecturer’s or professor’s email to change the grade. It all depends on how thorough you are with making research on the job.
Contact us at [[email protected]](mailto:[email protected]) for technical problems about your task or issue regarding hacking services.Change Your Grade Without Hacking the Whole School System
There will be no need to hack the whole database of the school if you can find a way to get into the mail of your professor or teacher (as the case maybe) especially if the grade has not been uploaded or updated on the central Database. This is less risky but still requires expertise. Know that these acts are also illegal.

• Time: – Know that time is very vital. The hacking operation should be done when no security review is going on. Most of the security reviews takes place in the day. This does not mean security cannot be reviewed through the night too. Also, you have to be fast during the operation. Every minute counts. Delay could lead to big problem.
• Method: – You need to be wary of your chosen method. By all means avoid using brute force or related method as it may crash the whole system (database) or reduce the load speed. This could reveal your intentions and would draw attentions to you.

Factors To Consider in Hacking Central Database

• Phishing – This is done by sending fake emails to obtain login information or other details so as to gain access to the email account or PowerSchool database. It is not 100% guaranteed to work.
• Brute Force: – This is done by using trial-and-error to guess login info, encryption keys, or access the unauthorised page. Hackers work through all possible combinations hoping to guess correctly.

CONCLUSION
To wrap it up, schools are using PowerSchool because it is more secured but it does not mean it cannot be hacked. Hacking platforms like PowerSchool requires great expertise, professional hacking tools and experience. It is usually advisable to employ the service of Jessica via email at [[email protected]](mailto:[email protected])

https://www.psychdb.com/teaching/biopsychosocial-case-formulation
Okay, my last therapist shared a different way to describe a patient's situation rather than a diagnostic label. I've read the article above and find this method dehumanizing and infuriating. Squeezing someone into 12 boxes, most of which are pathologized states. Like how the hell being poor is a pathology.
I insisted on no diagnosis, and that did not work. I don't want to be described in 12 boxes, this feels very insulting to me. Or perhaps if I ever go see that therapist again, I might suggest we first describe him by this table, than me, so that I don't feel abused.

Activation of Egg

Although fertilization is commonly considered solely the suggests that to mix 2 haploid nuclei, however it's AN equally necessary role in initiating the processes that begin development.

Definition:

A series of morphological, physiological, and molecular changes that occur within the egg in response to fusion of the spermatozoon with the egg is named is named Activation.
Activation of Egg Metabolism

Mechanism of Egg Activation:

All the activation events happen within the protoplasm and occur while not the involvement of the nuclei. The mature egg may be a metabolically inactive cell that's activated by the spermatozoon.The spermatozoon act sort of a massive secretion, binding to receptors on the egg membrane in all probability via G macromolecule that activate Srckinas, that activate PLC, that via IP3 and DAG increase animate thing factor IV add increase hydrogen ion concentration (alkaline) severally that activate egg.This flux of Ca across the eggs starts a programmed set of metabolic events.

G Protein:

G macromolecule conjointly called G ester binding macromolecule, ar a family of macromolecule that acts as a molecular switches within cell and ar concerned in transmittal signal from a spread of stimuli outside the cell to its interior.

Srckinase Function;

It is non receptor macromolecule amino acid enzyme,that ar concerned within the management of a spread of cellular method like,Proliferation,Differentiation,Motility andAdhesion.

Demographics: White, lived in New Jersey my whole life in a middle-upper-class family. I attend a very competitive high school: The average SAT is around 1450, and around 7/60 kids per grade get into Ivy League Schools
GPA: 3.87 UW/4.37 W
Class Rank: My school does not do class rank since it is a specialized high school for gifted teenagers.
Coursework: Every class I have taken at my high school is AP level, but it is not an AP class. In other words, it is not labeled as an AP class but I have taken three AP exams without studying for them and got 5's on all of them just by taking the class. I also have 12 college credits from taking certain classes throughout my high school career.
AP Tests: 5 on the AP chemistry test, 5 on the AP Environmental Science test, and 5 on the AP Human Geography test.
SAT and ACT: 1480 (780 Math/ 700 English), 32 ACT
Awards: Nothing notable, won a county science fair for an independent research project freshman year, and I have the biliteracy seal for Spanish.
ECs:

• Part-time job for three years
• Member of NHS
• 150+ Volunteer Hours
• Two poems published
• School Clubs:
  • Spanish Club (3 years)
    • Vice-President for 1 year
  • Focus On The Drive Club (3 years)
    • Treasurer for 1 year
  • Chemistry Olympics (1 year)
  • Literary Magazine (1 year)
  • Yearbook Club (2 years)
  • Baking Club (1 year)
  • Human Rights Club (1 year)

LOR and Essays: Great LORs from my chemistry, Spanish, and physics teachers. I have two essays that I believe are good, and have been proofread by every person I could get them proofread by. Examples are my family, my friends that are good at writing, and my 3 English teachers.
Intended Major: I would like to go for Aerospace Engineering
Chance Me: Georgia Tech and the University of Michigan are my main two, but also any other great schools you can think of for aerospace engineering would be helpful.

I'm trying to zinc-plate a steel part, however that part has alluminum that has been pressed on and cannot be removed.
I've read that vinegar plating zinc is a poor method of zinc plating due to a nonuniform coating and that it'll corrode the aluminum. What is an easily accessible (either to buy or produce) electrolyte that I could use?
From my basic chemistry knowledge it seems like ZnCl and ZnPO4 are not simple to make nor acquire but maybe I'm wrong? ZnOH also seems like it'll eat the aluminum. Maybe if I make a eat solution of ZnOH it won't eat the aluminum but will still allow for plating?

Some compilation footage of Mick Coup, a self-defence instructor. (Main Video Link: https://youtu.be/1m_jPckeqrc)
I didn’t want to succumb to stereotype but I definitely placed the ENTJ label on him immediately. I could say I felt all of this pointed towards attitudes that were obviously something whatever, Te-Se that..
But honestly, I just thought clear-cut confrontational vibes (maybe its just the nature of his job), focus on efficiency with realistic objective standards in mind, the scientific method all that, I guess an intuitive explanation of why things are the way they are. Physically built and imposing, commanding presence, British. If I said I didn’t just think “Gordon Ramsay ass mf” I’d be lying. Anyone could give a more accurate typing?